recombinant serpine2  (TargetMol)

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression is significantly upregulated in COAD. (A) Box plot of SERPINE family genes mRNA expression.in COAD(p < 0.05). (B) Box plot of SERPINE1 and SERPINE2 protein expression in COAD(p < 0.05). (C) Box plot of SERPINE2 mRNA expression among groups of different tumor grades in COAD (p < 0.05).

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression correlates with tumor immunoregulation, division and proliferation. (A) Volcano map of SERPINE2 co-expressed genes in COAD. (B, C) GO and KEGG enrichment analysis of SERPINE2 co-expressed genes obtained from the cBioPortal, the terms p and q < 0.05 were considered to be significantly enriched. Only 20 leading gene sets are displayed in the plot.

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is positively correlated with M2 macrophage infiltration in COAD. (A) Scatter plot showing the correlation of 6 different immune cell types and SERPINE2 expression (p < 0.05). The black line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (B) Scatter plot showing the correlation of CD68+ Marcophage and SERPINE2 expression (p < 0.05). C.Scatter plot showing the correlation of M1/M2 macrophage and SERPINE2 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (p < 0.05).

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is associated with infiltration of CD68 macrophage in COAD patients. (A) The expression of SEPRINE2 in patient tissues was detected by IHC. Adjacent tissue not expressing SERPINE2; tumor tissue strongly expressing SERPINE2. (B) Scatterplot showing the correlation between SERPINE2 and CD68 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the relationship between SERPINE2 and CD68 expression. (C) The expression of SERPINE2 and CD68 in patient tissues were detected by IHC. High levels of SERPINE2 expression are associated with increased expression of CD68. (* p<0.05, **p<0.01, *** p<0.001).

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: The external secretion of SERPINE2 from colorectal cancer cells promotes macrophage polarization to accelerate the development of colorectal cancer. (A) Schema diagram showing that THP-1 cells were treated with PMA, followed by the treatment of SERPINE2 recombinant protein as indicated treatments to obtain activated macrophages. Then tumor cells cocultured with these macrophages. were used to subsequent experiments. (B, C) RT-qPCR and Westen blot displayed SERPINE2 mRNA and Protein expression were upregulated in colorectal cancer cells. (D) The secretion of SERPINE2 in colorectal cancer cells was detected by ELISA. (E) The effect of SERPINE2 recombinant protein on macrophage polarization. (F) RT-qPCR displayed conditioned medium (CM) derived from colorectal cancer cells promotes the upregulation of macrophage M2 markers, compared to normal Intestinal cells. (G) ELISA analysis demonstrated that SERPINE2 secretion was significantly reduced after SERPINE2 knockdown. (H) RT-qPCR displayed conditioned medium with SERPINE2 knockdown down-regulated macrophage M2 markers. (I, J) The conditioned medium from macrophages treated with SERPINE2 recombinant protein promoted cancer cell proliferation (measured by CCK-8 assay) and migration (assessed by Transwell assay. (*p<0.05, **p<0.01, ***p<0.001).

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Recombinant, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Knockdown, CCK-8 Assay, Migration, Transwell Assay

Journal: Oncogene

Article Title: ZEB1-repressed microRNAs inhibit autocrine signaling that promotes vascular mimicry of breast cancer cells

doi: 10.1038/onc.2017.356

Figure Lengend Snippet: COPII-mediated secretion is essential for VM. ( a ) Quantitative reverse transcription–PCR (qRT–PCR) for SEC13 in MDA-MB-231 or BT-549 cells transfected with siNT or siSEC13. Error bars represent s.d. of three (MDA-MB-231) or two (BT-549) independent experiments. ( b ) Representative images of VM in MDA-MB-231 or BT-549 cells transfected with siNT or siSEC13. Scale bars=1000 μm. ( c ) Quantification of total network length in VM assays in MDA-MB-231 or BT-549 cells. Error bars represent s.d. for five or three independent experiments, respectively. T -tests were performed to compare each to siNT control, *** P <0.001, * P <0.05. ( d ) qRT–PCR following expression of a NEG or clustered miRNA mimics. Error bars represent s.d. of three independent experiments. T -tests were performed vs NEG control, *** P <0.001. ( h ) Western blot for FN1 and SERPINE2 in conditioned media from MDA-MB-231 cells transfected as shown. ( f ) Quantification of three (FN1) or two (SERPINE2) independent replicates of western blots as shown in e . Error bars represent s.d. T -tests were performed for FN1 quantitation compared to NEG control. ** P <0.05; *** P <0.001. ( g ) qRT–PCR following transfection of MDA-MB-231 cells with miRNA mimics. Error bars represent s.d. from three independent experiments. T -tests were performed, * P <0.05, ** P <0.01, *** P <0.001. ( h ) Western blot for LRP1 following transfection of MDA-MB-231 cells with miRNA mimics. Shown is a representative image of two independent experiments. GAPDH is loading control. ( i ) qRT–PCR following transfection of MDA-MB-231 cells with miRNA mimics. Error bars represent s.d. from three independent experiments. T -tests were performed, * P <0.05, *** P <0.001.

Article Snippet: Recombinant FN1 (1918-FN-02M, R&D Systems, Minneapolis, MN, USA) and SERPINE2 (2980-PI-010, R&D Systems) were added to VM assays at concentrations of 1 and 10 μg/ml, respectively.

Techniques: Reverse Transcription, Quantitative RT-PCR, Transfection, Control, Expressing, Western Blot, Quantitation Assay

Journal: Oncogene

Article Title: ZEB1-repressed microRNAs inhibit autocrine signaling that promotes vascular mimicry of breast cancer cells

doi: 10.1038/onc.2017.356

Figure Lengend Snippet: Increased expression of FN1 and SERPINE2 is regulated by serum withdrawal and is essential for VM. ( a ) Quantitative reverse transcription–PCR (qRT–PCR) in breast cancer cell lines cultured with 10 or 0% serum for 48 h. Error bars represent s.d. of three independent experiments. T -tests were performed for each line comparing 0vs10% conditions, ** P <0.01, *** P <0.001. ( b , d ) Representative images of VM in control and FN1 ( b ) or SERPINE2 ( d ) knockdown cells. Scale bars=1000 μm. ( c , e ) Quantification of total network length for VM assays as shown in b and d . Error bars represent s.d. for at five and four independent experiments, respectively. T -tests were performed to compare each to siNT control, ** P <0.01. ( f ) Representative images of VM in control and FN1 or SERPINE2 knockdown in BT-549 cells. Scale bars=1000 μm. ( g ) Quantification of total network length. Error bars represent s.d. for three independent experiments. T -tests were performed to compare each to siNT control, * P <0.05. ( h ) Representative phase images of cell morphology in siNT or siSERPINE2 cells. Scale bars=100 μm. ( i ) qRT–PCR for FN1 and CDH1 in cells transfected with siNT or siSERPINE2. Error bars represent s.d. of three independent experiments. T -test was performed, *** P <0.001. ( j ) qRT–PCR for FN1 and SERPINE2 in cells transfected with NEG or clustered miRNA mimics for 48 h, then cultured with 10 or 0.5% serum for 48 h. Error bars represent s.d. of three independent experiments. T -tests were performed comparing 0.5vs10% in the control or comparing cells transfected with miRNAs vs NEG in matching serum conditions, *** P <0.001. ( k ) Western blot for FN1 for cells as in h . ACTB is loading control. ( l , m ) Quantification of total network length of VM assays from VM-incompetent cells plated in 0% serum ( l ) or VM-competent cells plated in 5% serum ( m ) in the presence or absence of recombinant FN1 and SERPINE2 as shown. Error bars represent s.d. of two independent experiments.

Article Snippet: Recombinant FN1 (1918-FN-02M, R&D Systems, Minneapolis, MN, USA) and SERPINE2 (2980-PI-010, R&D Systems) were added to VM assays at concentrations of 1 and 10 μg/ml, respectively.

Techniques: Expressing, Reverse Transcription, Quantitative RT-PCR, Cell Culture, Control, Knockdown, Transfection, Western Blot, Recombinant

Journal: Oncogene

Article Title: ZEB1-repressed microRNAs inhibit autocrine signaling that promotes vascular mimicry of breast cancer cells

doi: 10.1038/onc.2017.356

Figure Lengend Snippet: Autocrine signaling factors FN1, ITGB1, SERPINE2 and LRP1 predict poor survival in early-stage breast cancer. ( a ) Plots comparing FN1 , ITGB1 , SERPINE2 and LRP1 levels in RNA-seq data from subtyped breast cancer cell lines. A one-way analysis of variance with Tukey’s test was performed, * P <0.5, *** P <0.001. ( b – f ) Survival curves from cBioPortal showing survival of patients with stage I, II or III breast cancer with the following genes altered. Log-rank test P -values are shown. ( b ) Tumors that had a low level (defined by heterozygous loss or homozygous deletion) of MIR200C along with high expression (defined by a gain, amplification or fold expression >2) of FN1 and SERPINE2 (red) compared to those that did not (blue). ( c ) Tumors that had a low level of MIR183 along with high expression of FN1 and SERPINE2 (red) compared to those that did not (blue). ( d ) Tumors that had a high level of FN1 , ITGB1 , SERPINE2 and LRP1 (red) compared to those that did not (blue). ( e ) Tumors that had a high level of FN1 and ITGB1 (red) compared to those that did not (blue). ( f ) Tumors that had a high level of SERPINE2 and LRP1 (red) compared to those that did not (blue).

Article Snippet: Recombinant FN1 (1918-FN-02M, R&D Systems, Minneapolis, MN, USA) and SERPINE2 (2980-PI-010, R&D Systems) were added to VM assays at concentrations of 1 and 10 μg/ml, respectively.

Techniques: RNA Sequencing, Expressing, Amplification